Analysis of seven micronutrients in breast milk of lactating mothers from the Central Region of Ghana using epithermal neutron activation

Abstract

Background: Breast milk contains various micronutrients which nourishes a baby with nutrition, and therefore the endorsement for exclusive breastfeeding during the first six months after birth. Such micronutrients, if in excess, can have adverse effects on the baby.

Objective: The levels of seven micronutrients in breast milk obtained from 27 lactating mothers in the Cape Coast Metropolitan area have been determined using Epithermal Neutron Activation Analysis (ENAA). This technique was used because it is suitable for performing both qualitative and quantitative multi-nutritional analyses on samples, and offers accuracies and sensitivities superior to those attainable by other methods.

Materials and Methods: During the analysis, a- 3 mm thick flexible boron was used to cut-off thermal neutrons so as to assess epithermal neutrons, thereby creating an activation energy which measured the levels of micronutrients in the breast milk. The standard reference materials used were the International Atomic Energy Agency (IAEA)-336; IAEA-407, IAEA-350 and National Institute of Standard and Technology (NIST) USA SRM 1577b. The Relative standardization method was used in the quantification of the micronutrients, with an accuracy of about 94.7 %. The micronutrients are Sodium (Na), Magnesium (Mg), Potassium (K), Calcium (Ca), Manganese (Mn), Copper (Cu) and Iodine (I).

Results: Except for iodine which had levels below the recommended dietary allowance (RDA), the remaining micronutrients had levels above the upper limit of the RDA, with Manganese being the highest.

Conclusion: The levels recorded are directly linked to the food intake of the mothers, and therefore the need for pregnant and lactating mothers to be mindful about what they eat. Children could be exposed to metabolic disorders and diseases as a result of such high levels.